Superfrost plus slides and standard slides can be silinized but I have found that superfrost slides work best so that protocol is outlined here.

Superfrost Plus Slides

TESPA-3 aminopropyltrioxysilane (Sigma # A3648)

acetone

EtOH

Concentrated HCl

• Dip a rack of superfrost plus slides in 2N HCl (200 ml Q + 40 ml concentrated HCl) for 1 minute.

• Rinse the slides for 1 minute in Milli Q water.

• Rinse the slides in 100% Acetone for 1 minute.

• Incubate in 100% Acetone containing TESPA for 1 minute.

• Rinse briefly in 100% acetone.

• Rinse briefly in 95% EtOH followed by 50% EtOH, 30% EtOH and several changes of Q.

• Air dry at room temperature overnight and then store at 4° dessicated until use. They keep for several months.